计算溶液所需的质量、体积或浓度。
BioReagent,无菌,用于聚丙烯酰胺凝胶电泳,用于蛋白分析,适用于电泳 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Laemmli蛋白上样缓冲液 (4×, 还原型) 是一种以溴酚蓝为染料的4倍浓缩的蛋白上样缓冲液,主要由β-巯基乙醇、SDS、溴酚蓝等组成,可用于不连续蛋白电泳的上样。
该试剂仅用于科研领域,不适用于临床诊断或其他用途。
操作步骤(仅供参考):
1、取适量的蛋白样品和Laemmli蛋白上样缓冲液 (4×, 还原型) 按3:1混合,充分混匀。如 60 μL 蛋白 + 20 μL Laemmli蛋白上样缓冲液 (4×, 还原型);
2、直接上样到SDS-PAGE胶加样孔内即可;
3、通常电泳至蓝色染料到达PAGE胶的底部附近即可停止。
注意事项:
1、Laemmli蛋白上样缓冲液 (4×, 还原型) 中含少量β-硫基乙醇,有轻微刺激性气味。
2、为了您的安全和健康,请穿实验服并戴一次性手套操作。
3、试剂开封后请尽快使用,以防影响后续实验效果。
Laemmli Protein Sample Buffer (4×, Reducing) is a 4-fold concentrated protein loading buffer containing bromophenol blue as the tracking dye. It consists mainly of β-mercaptoethanol, SDS and bromophenol blue, and is used for sample loading in discontinuous protein electrophoresis.
This reagent is for research use only and is not intended for clinical diagnosis or other applications.
Operating Procedure (for reference only):
1. Mix an appropriate amount of protein sample with Laemmli Protein Sample Buffer (4×, Reducing) at a volume ratio of 3:1 and mix thoroughly. For example, 60 μL protein + 20 μL Laemmli Protein Sample Buffer (4×, Reducing).
2. Load the mixture directly into the sample wells of the SDS-PAGE gel.
3. Stop electrophoresis generally when the blue tracking dye migrates near the bottom of the PAGE gel.
Precautions:
1. Laemmli Protein Sample Buffer (4×, Reducing) contains a small amount of β-mercaptoethanol with a slight pungent odor.
2. For your safety and health, wear a lab coat and disposable gloves during operation.
3. Use the reagent as soon as possible after opening to avoid affecting subsequent experimental results.
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