计算溶液所需的质量、体积或浓度。
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存,避光。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
Product introduction：
L-Photo-Leucine is an analog of L-leucine that can be incorporated into proteins during synthesis and then light-activated to covalently crosslink interacting proteins in cells.
Features of L-Photo-Leucine:
• In vivo labeling—incorporate photo-reactive group into proteins using normal cellular machinery of live cells
• In vivo crosslinking—find interacting proteins in the native cellular environment
• Increased specificity—crosslink interacting proteins correctly positioned at their interfaces within protein interaction domains
• Efficient recovery—90% protein recovery in cell lysates after crosslinking
• Compatible—crosslink proteins expressed in a wide variety of cell lines, including HeLa, 293T, COS7, U2OS, A549, A431, HepG2, NIH 3T3 and C6
• Easy to use—reagents are photo-stable under normal laboratory lighting, eliminating the need to work in the dark
L-Photo-Leucine is an analog of L-leucine that can be endogenously incorporated into the primary sequence of proteins during synthesis and then ultraviolet light (UV)-activated to covalently crosslink proteins within protein-protein interaction domains in their native environment within live cells. The powerful method enables both stable and transient protein interactions in cells to be studied and characterized without the use of completely foreign chemical crosslinkers and associated reagent solvents during the interaction experiment that can adversely aspects of the cell biology being studied.
When used in combination with specially formulated limiting media that is devoid of leucine, the photo-activatable derivative is treated like a naturally occurring amino acid by the protein synthesis machinery within the cell. As a result, it can be substituted for leucine in the primary sequence of proteins during catabolism and growth. Photo-leucine derivatives contain diazirine rings that activate when exposed to UV light to become reactive intermediates that form covalent bonds with nearby protein side chains and backbones. Naturally associating binding partners within the cell can be instantly trapped by photoactivation of the diazirine-containing proteins in the cultured cells. Crosslinked protein complexes can be detected by decreased mobility on SDS-PAGE followed by western blot detection, size exclusion chromatography, sucrose density gradient sedimentation or mass spectrometry.
Resources:
Photoreactive Amino Acid FAQ
• Reactive Moiety:Diazirine
• Crosslinker Type:Heterobifunctional
• Form:Solid
• Spacer:Short(<10 Å)
• Labeling Method:Chemical Labeling,Metabolic Labeling
• Spacer Arm Length:0.0 Å
Features of L-Photo-Leucine:
• In vivo labeling—incorporate photo-reactive group into proteins using normal cellular machinery of live cells
• In vivo crosslinking—find interacting proteins in the native cellular environment
• Increased specificity—crosslink interacting proteins correctly positioned at their interfaces within protein interaction domains
• Efficient recovery—90% protein recovery in cell lysates after crosslinking
• Compatible—crosslink proteins expressed in a wide variety of cell lines, including HeLa, 293T, COS7, U2OS, A549, A431, HepG2, NIH 3T3 and C6
• Easy to use—reagents are photo-stable under normal laboratory lighting, eliminating the need to work in the dark
L-Photo-Leucine is an analog of L-leucine that can be endogenously incorporated into the primary sequence of proteins during synthesis and then ultraviolet light (UV)-activated to covalently crosslink proteins within protein-protein interaction domains in their native environment within live cells. The powerful method enables both stable and transient protein interactions in cells to be studied and characterized without the use of completely foreign chemical crosslinkers and associated reagent solvents during the interaction experiment that can adversely aspects of the cell biology being studied.
When used in combination with specially formulated limiting media that is devoid of leucine, the photo-activatable derivative is treated like a naturally occurring amino acid by the protein synthesis machinery within the cell. As a result, it can be substituted for leucine in the primary sequence of proteins during catabolism and growth. Photo-leucine derivatives contain diazirine rings that activate when exposed to UV light to become reactive intermediates that form covalent bonds with nearby protein side chains and backbones. Naturally associating binding partners within the cell can be instantly trapped by photoactivation of the diazirine-containing proteins in the cultured cells. Crosslinked protein complexes can be detected by decreased mobility on SDS-PAGE followed by western blot detection, size exclusion chromatography, sucrose density gradient sedimentation or mass spectrometry.
Resources:
Photoreactive Amino Acid FAQ
• Reactive Moiety:Diazirine
• Crosslinker Type:Heterobifunctional
• Form:Solid
• Spacer:Short(<10 Å)
• Labeling Method:Chemical Labeling,Metabolic Labeling
• Spacer Arm Length:0.0 Å
规格
别名
L-光亮氨酸
英文名称
L-Photo-Leucine
储存条件
2-8°C储存,避光
运输条件
低温运输
名称和识别符
分子类型
小分子
分子量
143.15
技术文档
高级数据
产品属性
| Reactivity | Nonselective-NA (Metabolic labeling) |
|---|
化学和物理性质
溶解性
Water
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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