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224,000+ 科研产品 · 三重ISO认证 · 每个产品均提供COA和SDS · 现货产品当日发货

1kb Ladder

Name: 1kb Ladder
SKU: L665551
Availability: InStock

货号: L665551

COA

有货

储存条件

-20°C储存

运输条件

超低温运输

规格

库存

价格

数量

100T

L665551-100T

期货

¥147.90

500T

L665551-500T

期货

¥582.90

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概述规格名称和识别符技术文档高级数据质检证书（CoA,COO,BSE/TSE 和分析图谱) 技术文档和文章溶液计算器

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为什么选择此级别

，适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-20°C储存。超低温运输。请查阅批次 COA 获取详细规格。

📋

质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

概述

1 kb Ladder由8条DNA片段组成，分别为1 kb、2 kb、3 kb、4 kb、5 kb、6 kb、8 kb和10 kb。本产品为已含有1×Loading Buffer的DNA溶液，可取5 μl直接电泳，使用十分方便；电泳时5 kb的DNA片段量约为150 ng，显示亮带，其他条带的DNA量约为50 ng。

组分	L665551 100 preps	L665551 500 preps
1 kb Ladder	500 µl	5×500 µl

注意事项：

1．本产品可直接化冻混匀使用，无需加热。

2．请及时更换电泳缓冲液并使用新制琼脂糖凝胶，以免影响电泳结果。

3．DNA琼脂糖凝胶电泳时，凝胶的纯度对DNA条带的清晰度影响很大，因此尽量选用质量好的琼脂糖，推荐凝胶浓度为0.7%-1%，电压4-8 v/cm。

4．凝胶浓度与DNA片段的分离性能关系密切。凝胶浓度越大，对短片段DNA分离性能越好；反之，凝胶浓度越小，越有利于长片段DNA的分离。

5．当与大分子染料配合使用时，建议适当减少Marker的用量或加大染料的用量。

使用方法：

取5 μl加入琼脂糖凝胶的加样孔中（如果加样孔较宽，大于6 mm时，须适当增加上样量），进行电泳。

实验结果：

0.7%琼脂糖凝胶电泳图(5 μl)

The 1 kb Ladder consists of 8 DNA fragments, namely 1 kb, 2 kb, 3 kb, 4 kb, 5 kb, 6 kb, 8 kb, and 10 kb. This product is a DNA solution that already contains 1 x Loading Buffer, which can be taken as 5 μ Direct electrophoresis is very convenient to use; The amount of 5 kb DNA fragments during electrophoresis is about 150 ng, showing bright bands, while the amount of DNA in other bands is about 50 ng.

Component	L665551 100 preps	L665551 500 preps
1 kb Ladder	500 µl	5×500 µl

Notes:
1. This product can be directly thawed and mixed for use without heating.
2. Please replace the electrophoresis buffer in time and use the newly prepared agarose gel to avoid affecting the electrophoresis results.
3. During DNA agarose gel electrophoresis, the purity of gel has a great impact on the clarity of DNA bands, so try to use high-quality agarose. The recommended concentration of gel is 0.7% -1%, and the voltage is 4-8 v/cm.
4. The concentration of gel is closely related to the separation performance of DNA fragments. The higher the concentration of gel, the better the separation performance of short DNA fragments; On the contrary, the smaller the gel concentration, the more conducive to the separation of long DNA fragments.
5. When used in combination with macromolecular dyes, it is recommended to appropriately reduce the amount of Marker or increase the amount of dye used.

Usage:
Take 5 μ L Add it into the sample adding hole of agarose gel (if the sample adding hole is wider than 6 mm, the sample loading amount must be increased appropriately), and conduct electrophoresis.
Experimental results:

0.7% agarose gel electrophoresis (5 μ L)