计算溶液所需的质量、体积或浓度。
BioReagent,生物染色剂,用于显微镜 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
真菌属于真核生物,没有质体,营养方式为吸收,无吞噬作用,通常其细胞壁含有甲壳质和β-葡聚糖。可引起人类、动物的疾病,称为真菌病,还可引起植物的疾病、人类过敏性疾病和真菌毒素中毒症。在医学领域,为了了解真菌的结构,通常需要对载有真菌的玻片进行染色。现有的而普通封片胶(中性树脂)的方法只适用于干片的封片,无法对染色后的湿片进行封片,适用范围受到了限制,且染色后的保持时间较短。
霉菌 (molds) 是形成分枝菌丝的真菌的统称,意即"发霉的真菌",它们往往能形成分枝繁茂的菌丝体,但又不象蘑菇那样产生大型的子实体。霉菌菌丝较粗大、孢子溶于在空气中飘散,所以制标本时常用乳酸酚棉蓝染色液。
乳酸酚棉蓝染色液常用于烟曲霉菌等染色,其特点是不会使细胞变形,能够杀菌,并且不易干燥,保持很长时间。本产品仅适用于科研实验,不可做他用。
使用说明(仅供参考):
1、涂片:载玻片编号,于载玻片中央滴加2~3滴乳酸酚棉蓝染色液。
2、用灭菌接种环或牙签等器具取一小块有颗粒或颜色部分真菌菌落。
3、放入载玻片上的乳酸酚棉蓝染色液中,混匀。
4、加盖洁净的盖玻片,轻轻按压制成压片。
5、光学显微镜在低倍、高倍或油镜下观察真菌形态。
注意事项:
1、涂片之前应事先在背面做好圆圈标记,以便判断后续试验的位置。
2、取细菌时,应注意自我防护。该试剂均对人体有刺激性,请注意适当防护。
3、待检细菌培养时间也会影响染色,阳性菌培养时间过长或已死亡或细菌溶解,都常呈阴性反应。
4、为了您的安全和健康,请穿实验服并戴一次性手套操作。
染色结果:
| 真菌(尤其是烟曲霉菌)中孢子与菌丝 | 蓝色 |
| 背景 | 淡蓝色 |
Fungi are eukaryotes devoid of plastids, with an absorptive nutritional mode and no phagocytosis. Their cell walls generally contain chitin and β-glucan. Fungi can trigger illnesses in humans and animals known as mycoses; they also cause plant diseases, human allergic disorders, and mycotoxicoses. In medical research, staining slides carrying fungal samples is a standard practice to visualize fungal structures. Conventional neutral resin mounting medium is only suitable for dry slide mounting and cannot be applied to stained wet specimens, limiting its application scope. Additionally, stained slides prepared with this medium have a short retention period.
Molds are a general term for fungi that produce branched hyphae, literally meaning "mold-forming fungi". They often develop extensively branched mycelium but do not generate large fruiting bodies like mushrooms. Mold hyphae are relatively thick, and their spores disperse readily in the air, so lactophenol cotton blue staining solution is widely used for specimen preparation.
Lactophenol cotton blue staining solution is commonly employed for staining Aspergillus fumigatus and other fungi. It preserves cellular morphology without distortion, possesses bactericidal properties, resists desiccation, and enables long-term slide storage. This product is intended solely for scientific research and shall not be used for other purposes.
Instructions for Use (For Reference Only):
1. Smear preparation: Label the glass slide, and drop 2–3 drops of lactophenol cotton blue staining solution onto the center of the slide.
2. Use a sterile inoculating loop or toothpick to collect a small fragment of fungal colony containing granules or pigmented regions.
3. Transfer the collected fungal material into the lactophenol cotton blue staining solution on the slide and mix thoroughly.
4. Cover with a clean coverslip and press gently to create a squash mount.
5. Observe fungal morphology under a light microscope at low magnification, high magnification, or oil immersion objective.
Precautions:
1. Draw a circular mark on the reverse side of the slide prior to smear preparation to locate the specimen area for subsequent experiments.
2. Wear appropriate personal protective equipment while collecting fungal samples. This reagent is irritating to human tissues, so adequate protection is mandatory.
3. Incubation duration of fungal cultures affects staining outcomes. Over-aged, dead, or lysed fungal cultures frequently yield false-negative staining results.
4. Wear a lab coat and disposable gloves throughout all operations for personal safety and health protection.
Staining Results
| Spores and hyphae of fungi (especially Aspergillus fumigatus) | Blue |
| Background | Pale blue |
通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | L774540 | |
| 分析证书 | L774540 | |
| 分析证书 | L774540 | |
| 分析证书 | L774540 | |
| 分析证书 | L774540 | |
| 分析证书 | L774540 | |
| 分析证书 | L774540 | |
| 分析证书 | L774540 |
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 BioReagent 级别指南 → 查看 Biological Stain 级别指南 → 查看 for Microscopy 级别指南 →