Synergistic promotion of sludge reduction by surfactant-producing and lysozyme-producing bacteria: Optimization and effect of Na+

To improve the efficiency of aerobic digestion, this investigation utilized the synergistic effect of lysozyme-producing strain YH14 and surfactant-producing strain ZXY4 to promote sludge hydrolysis, and added NaCl to enhance this promoting effect. The best performance in promoting sludge hydrolysis was achieved when the inoculum of functional bacteria was 12 % (inoculum ratio of strain YH14: strain ZXY4 = 1:3) and the dosage of NaCl was 5 g L -1 , which caused an increase of 19.25 % in the SS removal rate and 2588.21 mg L -1 in the SCOD release, as compared with the control. Fluorescence region integral analysis shows that the synergy of two functional bacteria and NaCl can enhance the biodegradability of sludge. Protein secondary structure analysis shows that strain ZXY4 and Na + cause the EPS structure to loosen, increasing the chances of lysozyme lysis of bacteria. Nucleotide metabolism, metabolism of other amino acids and membrane transport enhanced in a co-processing system.