摘要
In this study, a pure Grifola frondosa polysaccharide (GFP-1) was extracted and purified from Grifola frondosa . By HPLC , GC-MS, FT-IR, and NMR analysis, GFP-1 was determined to be a starch-like polysaccharide with an average molecular weight of 3370 kDa. It included three monosaccharides , i.e., glucose, galactose , and mannose . The backbone of GFP-1 consisted of →4)-α-Glc p -(1→ and →4,6)-α-Glc p -(1 → . The side branches were composed of →6)-α-Gal p -(1→, α-Glc p -(1→, and a small amount of α-Man p -(1 → . By using a cyclophosphamide (CTX)-induced immunosuppressed mice model, we evaluated the immunomodulatory activity of GFP-1. The results showed that GFP-1 increased the thymic and spleen indices, promoted the level of IgG and IgA in serum, and activated the mitogen-activated protein kinase (MAPK) pathway in CTX-induced mice. Also, GFP-1 significantly promoted the mRNA expression of intestinal barrier factors and protected intestinal structural integrity in immunosuppressed mice. In conclusion, the data presented here suggested that GFP-1 might be a potential immune-enhancing supplement.
危险品化学品经营许可证（带存储）