A novel nanocomposite material C3F7-azo+/Ti4O92− was prepared as a sensor for the detection of ascorbic acid and uric acid

A novel layered nanocomposite, C3F7-azo+/Ti4O92−, was prepared using the stripping/restacking method with K2Ti4O9 as the raw material and trans-[2-(2,2,3,3,4,4,4-heptafluorobutylamino) ethyl]-{2-[4-(4-hexyphenylazo) -phenoxy] ethyl}-dimethylammonium (hereinafter referred to as C3F7-azo+Br−) as the modifier. After modification on glassy carbon electrodes (GCE), it was used for simultaneous detection of ascorbic acid (AA) and uric acid (UA). The morphology and elemental composition of the nanocomposites were characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), and energy dispersive X-ray spectroscopy (EDS). The electrochemical properties were measured and analyzed by differential pulse voltammetry (DPV), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). Compared to single-component modified electrodes (K2Ti4O9/GCE, C3F7-azo+/GCE), the layered nanocomposite modified electrode (C3F7-azo+/Ti4O92−/GCE) exhibited a larger electrochemically active area (0.074 cm2), lower charge transfer resistance (94.0 Ω), and greater catalytic activity toward AA and UA. The detection limits were 0.39 μM for AA and 0.75 μM for UA, within the concentration ranges of 1.20–26.30 μM and 0.94–20.41 μM, respectively. The efficacy of C3F7-azo+/Ti4O92− was also demonstrated in real rabbit serum samples, with recoveries between 96.1% and 104.2%.