Green in situ immobilisation of gold nanoparticles on bacterial nanocellulose membranes using Tannic acid and its detection of Fe3+

Oxidative stress is one of the factors that promote melanogenesis . Trivalent iron ions play a key role in regulating the iron-catalysed oxidative stress response. A novel SERS flexible membrane sensor based on tannic acid with good sensitivity and uniformity was prepared by green in situ reduction of gold nanoparticles on bacterial cellulose membrane（BCM）with a simple and highly selective method to detect Fe 3+ . Under alkaline conditions, Fe 3+ is adsorbed on the BCM-TA@Au NPs flexible membrane by tannic acid (TA) through chelation, thus enabling the detection of Fe 3+ . Furthermore, this simple detection system has a wide linear detection range and high sensitivity to effortlessly evaluate Fe 3+ at concentrations up to 10 −7 M. More importantly, the proposed SERS flexible substrate performed well in determining Fe 3+ concentrations in B16 melanocytes , providing new insights into the factors affecting the melanin synthesis pathway and providing a potential biomarker for melanoma treatment.