Dual-responsive fluorescence probe for measuring HSO3− and viscosity and its application in living cells and real foods

Microenvironmental indicators in organisms drive the operation of different physiological functions. In contrast, disruption of microenvironmental homeostasis is often closely associated with various pathological processes. A novel dual-response fluorescent probe based on hemicyanine dye ( HT-Bzh ) was designed and synthesized for the detection of HSO 3 − and viscosity changes. The probe not only provides high sensitivity (limit of detection = 0.2526 μM) for the detection of HSO 3 − using the Michael addition reaction, but also allows the observation of fluorescence emission at 528 nm and thus the monitoring of viscosity changes through hindering of the twisted intramolecular charge transfer (TICT) mechanism. Additionally, dual-response probe has been successfully used to image living cells and detect real food samples. As a new designed tool, HT-Bzh shows excellent anti-interference capability and biocompatibility, which makes it have application potential in other biological systems and in-vivo imaging.