A Novel Electrochemiluminescence Modified Glasss Carbon Electrode Based on Carboxylated Acetylene Black / Ru(bpy)32+ / Nafion for Detection of Isoniazid

A novel, rapid, and facile electrochemiluminescence sensor which based on carboxylated acetylene black / Ru(bpy) 3 2+ / nafion modified glass carbon electrode (CAB/Ru(bpy) 3 2+ /NAF/GCE) was developed for detecting isoniazid (INH). The CAB/Ru(bpy) 3 2+ /NAF/GCE expresses an efficient electrochemiluminescence response for INH, because of the brilliant luminous efficiency of Ru(bpy)32+ and admirable electronic transmission of acetylene black. The electrochemiluminescence intensity was quenched significantly with addition of INH. Under the optimal conditions, the sensor for the detection of isoniazid exhibits a wide linear range of 1.0 × 10 -6 ~ 1.0 × 10 -3 mol·L -1 with the detection limit of 5.0 × 10 -7 mol·L -1 . The sensor is expected to be a simple and convenient method for the determination of INH in pharmaceutical samples .