Signal Amplification of Self-Potential Biosensor for Glucose Monitoring

A self-powered electrochemical sensor (SPS) has been designed for sensitive detection of glucose based on the glucose oxidase (GOD)/polyvinyl ferrocene (PVF)/ poly(diallyldimethylammonium chloride) (PDDA -reduced graphene oxide (RGO)/glassy carbon electrode (GCE) as a bioanode and bilirubin oxidase (BOD)/PDDA-RGO/GCE as a biocathode. The signal amplification of electron mediator in the electrochemical anode has been investigated in details. The results exhibited that the electron mediator did amply the signal of SPS and the mediator existed on the surface of electrode was better than that existed in a solution. The electrodes constructed under the optimal conditions have 2 been used to develop a glucose SPS which showed a wide linear range from 0.1 mM to10 mM (R 2 =0.997) and a low detection limit of 23.7 μM (S/N=3). Therefore, the newly developed SPS was sensitive and facile for glucose detection, which could promise the possibility of its application in real samples.