A Lable-free ECL Biosensor for The Detection of Uric Acid Based on Au NRs@TiO2 Nanocomposite

A novel ultrasensitive electrochemiluminescence (ECL) biosensor was developed to detect uric acid (UA) based on Au NRs@TiO 2 nanocomposite. The Au NRs@TiO 2 nanocomposite was successfully prepared. The morphologies of Au NRs@TiO 2 nanocomposite were observed by scanning electron microscope (SEM), transmission electron microscopy (TEM). Morphological representation results showed that the nanomaterials were well dispersed and had a uniform surface. The characterization of electrochemiluminescence properties indicated that the Au NRs@TiO 2 possessed good ECL behavior. The Au NRs@TiO 2 nanocomposite was used to establish a highly sensitive electrochemiluminescence biosensor for the uric acid. A highly sensitive and selective electrochemiluminescence biosensor was established successfully which based on the Au NRs@TiO 2 nanocomposite. Under the optimal conditions, the biosensor shown a wide calibration range from 40 nM to 28 μM (R = 0.9985) with detection limit of 15 nM. The proposed electrochemiluminescence biosensor provided selective detection of uric acid range from 40 nM to 28 μM (R = 0.9985) with detection limit of 15 nM. This biosensor exhibited stable, sensitive and reproducible detection of uric acid, and was able to be used in clinical detection.