The Novel biocatalytic cascade ZIF-8 capsule/Polysulfone Stereostructure and its Application in Amperometric Glucose Biosensors

In this study, we present a novel zeolitic imidazolate framework-8 metal-organic framework /polysulfone (PSF) stereostructure with glucose oxidase (GOx) and horseradish peroxidase (HRP) encapsulated in ZIF-8 as nanoreactors. This work links the high biocatalytic activity and great stability of the enzymes encapsulated in ZIF-8 with the large surface area and mass transport of the threedimensional microporous PSF and highlights the pore structure of PSF, which blocks cells during whole blood testing. The prepared ZIF-8 capsule/PSF structure exhibits a 1.2-fold and 2.4-fold increase in sensitivity compared with the ZIF-8 capsule powder and bulk mixture of GOx and HRP, respectively. The modified ZIF-8 capsule/PSF glucose biosensor exhibited a 0-18 mM linear range and good selectivity. Furthermore, the prepared sensor attenuated less than 10% after 50 replicate whole blood tests. These advanced properties give the novel biocatalytic cascade ZIF-8 capsule/polysulfone stereostructure promising applications in whole blood monitoring.