Quantitative Determination of Kaempferol by using a novel B-Z Chemical Oscillating system Catalyzed by a Cu (II)-tetraazamacrocyclic Complex

An appropriate analytical technique for determination of kaempferol (KMF) by its perturbation effect on Belousov-Zhabotinskii (B-Z) oscillating system was reported. The macrocyclic copper (II) complex [CuL] (ClO 4 ) 2 ] was used as a catalyst while malic acid as substrate in B-Z system. The ligand L in the macrocyclic-complex is 5, 7, 7, 12, 14, 14-hexamethyl-1, 4, 8, 11-tetraazacyclotetradeca-4, 11-diene. Experimental outcomes shown the perturbation effect of KMF could cause the change in the amplitude of oscillation (ΔA) which was directly related to its concentration (2.5 × 10 -6 to 3.0 × 10 -4 mol/L) with correlation coefficient of 0.99315. The calculated relative standard deviation (RSD) is 3.1 % by eight samples (1.8 × 10 -5 mol/L) and the observed lower limit of detection is 1.25 × 10 -6 mol/L. The cyclic voltammetry (CV) experiments were used to confirm the redox reaction between kaempferol and sodium bromate. Furthermore, the reaction perturbation mechanism was derived from the well-known FKN (Field-Koros-Noyes) oscillation mechanism.