In situ synthesized eRAFT polymers for highly sensitive electrochemical determination of AFB1 in foods and herbs

An electrochemical sensor based on environmentally friendly eRAFT polymerization was developed for the detection of aflatoxin B 1 (AFB 1 ) in food and herbal medicine. Two biological probes, aptamer (Ap) and antibody (Ab), were used to specifically recognize AFB 1 , and a large number of ferrocene polymers were grafted on the electrode surface by eRAFT polymerization, which greatly improved the specificity and sensitivity of the sensor. The detection limit of AFB 1 was 37.34 fg/mL. In addition, the recovery rate was 95.69% to 107.65% and the RSD was 0.84% to 4.92% by detecting 9 spiked samples. The delighted reliability of this method was verified by HPLC-FL.