Use of hydrogen peroxide to prime the autohydrolysis and enzymatic hydrolysis efficiency of wheat straw pulp residues

Exogenous ash penalties cause straw pulp residues to exhibit low autohydrolysis performance due to an organic–inorganic colloid medium. In this study, H 2 O 2 was applied following the soil organism to oxidize the organic components playing a role in the buffer system by increasing the negative charge on the surface of the particulate ash, thus reducing the acid buffer and cation exchange capacity of the pretreated wheat straw pulp residue (WSPR). The results showed that with the enzymatic efficiency increasing concentrations (0–3%) of H 2 O 2 , the enzymatic efficiencies of pretreated WSPR improved significantly with increasing H 2 O 2 concentration (0–3%) from 49.73 to 88.27%. A linear correlation was observed between these increases and the accessibility of the substrate to the enzymes, the substrate's surface charge, and the lignin's available surface area. The addition of 2% H 2 O 2 in WSPR autohydrolysis was able to increase the production of glucose and xylooligosaccharides by 1.58 and 2.38 times, respectively. This work demonstrated an innovative option for producing fermentable sugars from WSPR by effectively abolishing the self-buffering effects during autohydrolysis.