Design fabrication of electrochemical sensor based on Ru(bpy)22+/SMWCNTs/Au/GCE electrode for the selective determination of 5′-guanosine monophosphate

5′-Guanosine monophosphate (5′-GMP) is one main source of freshness in broths. Herein, an electrochemical platform based on a novel ternary nanocomposite glassy carbon electrode modified with advantageously-united gold nanoparticles, 2,2′-bipyridine hydrated ruthenium (Ru(bpy) 2 Cl 2 ) and sulfonated multi-walled carbon nanotubes (SMWCNTs)was prepared and used to detect 5′-GMP. After conditions optimization, the best performance of the electrochemical sensor was found in acidic media, including high specificity, sensitivity and selectivity. The electrochemical sensor exhibited a wide linear range under the optimal conditions. The enhanced sensitivity of this sensor was attributed to the Ru(bpy) 2 Cl 2 and functionalized SMWCNTs that provided high electrical conductivity and electrocatalytic properties during electrochemical reaction . Precise analysis of 5′-GMP in actual broth samples showed satisfactory recovery. Thus, the sensor can be used in the market and food enterprises.