Cycloartane triterpene glycosides from Actaea vaginata

Phytochemical investigation of the whole plant of Actaea vaginata led to the isolation of five cycloartane triterpene glycosides , including three new Actaeoside A−C ( 1 − 3 ) along with two known analogues, beesiosides E ( 4 ) and N ( 5 ). Their structures were elucidated by NMR , HRESIMS and chemical analysis. The cytotoxicity of compounds ( 1 − 5 ) was assayed against HT-29 and HepG2 cancer cell lines. All the isolates showed moderate degrees of inhibitory activity against the HT-29 with IC 50 values in a range of 18.2–27.8 μM.