Multifunctional barrier membranes promote bone regeneration by scavenging H2O2, generating O2, eliminating inflammation, and regulating immune response

The injured bone microenvironment is typically characterized by high-level reactive oxygen species (ROS, mainly H 2 O 2 ) and low content of oxygen. The former can cause cell apoptosis, induce immune imbalance and inflammation, inhibit osteogenic differentiation, and delay bone repair . In this work, barrier membranes made of a combination of hydroxyapatite nanowires (HAp NWs) and polylactic acid (PLA) loaded with manganese dioxide nanosheets (NSs) ( [email protected] ) were prepared to guide bone regeneration (GBR). Due to the catalytic function of MnO 2 , [email protected] membranes are able to effectively decompose H 2 O 2 to generate O 2 . In vitro experiments demonstrated [email protected] membranes inhibited the apoptotic process caused by high level of H 2 O 2 , decreased intracellular oxidative stress , suppressed inflammation-related gene/protein expression, and inhibited macrophage M1 polarization. The nanostructured HAp stimulated osteogenic differentiation, and the loading of MnO 2 NSs played a synergistic effect. In addition, the barrier function of PLA could hamper the epithelium overgrowth, providing the development space for periodontal regeneration. By scavenging H 2 O 2 , generating O 2 , eliminating inflammation, and regulating immune response, [email protected] membranes efficiently promoted bone regeneration. Our results confirmed that composite membranes have great potential for application in GBR, and provide a reference for future design of bone repair materials.