Rapid detection of kanamycin using cooperative recognition split aptamer and graphene oxide nanosheets

Split aptamers are fragments of the parent aptamer which still possess binding ability to its target. They have shown great potential in the development of aptasensors. In this study, a fluorescence aptasensor was constructed employing the split aptamer as a cooperative recognition molecular probe and Graphene Oxide nanosheets (GO) as a quencher for rapid detection of kanamycin in chilled pork. Under optimal conditions,the proposed biosensor showed a good linear relationship in the range of 0.5–50 nM with the detection limit of 0.36 nM. The recoveries of the actual samples ranged from 97.6 to 103.5%. Furthermore, the test can be completed in 25 min. This study provides good reference for developing simple, economical, and rapid assay for other small molecular targets detection.