摘要
There is a significant clinical demand for bone repair materials with high efficacy. This study was designed to fabricate nanofibrous scaffolds to promote bone defect regeneration using magnesium doped mesoporous bioactive glass (MBG), a fusion protein Osteocalcin-Osteopontin-Biglycan (OOB), silk fibroin (SF) and nerve growth factor (NGF) for facilitating accelerated bone formation. We found that MBG adsorbed with OOB ( [email protected] ) as core, and SF adsorbed with NGF ( [email protected] ) as shell to fabricate the nanofibrous scaffolds ( [email protected] / [email protected] ) through coaxial electrospinning. [email protected] / [email protected] scaffolds could effectively mimic the component and structure of bone matrix. Interestingly, we observed that [email protected] / [email protected] scaffolds could substantially promote bone mesenchymal stem cells (BMSCs) osteogenesis through stimulating Erk1/2 activated Runx2 and mTOR pathway, and it could also activate the expression level of various osteogenic marker genes. Intriguingly, [email protected] / [email protected] scaffolds could also enhance BMSCs induced neural differentiation cells differentiated into neuron, and activate the expression of the different neuron specific marker genes. Moreover, it was found that [email protected] / [email protected] scaffolds accelerated bone regeneration with neurogenesis, and new neurons were formed in Haversian canal in vivo . Consistent with these observations, we found that Erk1/2 and mTOR signaling pathways also regulated osteogenesis with the neurogenesis process from RNA sequencing result. Overall, our findings provided novel evidence suggesting that [email protected] / [email protected] scaffolds could function as a potential biomaterial in accelerating bone defect regeneration with neurogenesis, as well as in recovering the motor ability and improving the quality of life of patients.
危险品化学品经营许可证（带存储）