Enhanced degradation of atrazine through UV/bisulfite: Mechanism, reaction pathways and toxicological analysis

Atrazine residue in the environment continues to threaten aquatic ecosystem and human health owing to its adverse effect. However, limited researches focused on degradation mechanism of atrazine by UV/bisulfite, especially risk of intermediates at cellular and molecular level has not been seriously elaborated. In current work, transformation patterns and residual toxicity of intermediates of atrazine by UV/bisulfite were systematically investigated. The atrazine degradation was described by a pseudo first-order kinetic model ( K obs = 0.1053 min −1 ). The presence of H 2 PO 4 − , HCO 3 − and HA had a powerful inhibition. Scavenging test of radicals illustrated that SO 4 •− , •OH and O 2 •− existed in UV/bisulfite system, SO 4 •− and •OH were mainly responsible for atrazine degradation. Eight degradation intermediates were identified, which were involved in dealkylation, alkyl oxidation, dechlorination-hydroxylation, and alkylic-hydroxylation. E. coli as a model microorganism was selected to assess the risk of degradation intermediates. The levels of reactive oxygen species , MDA and Na + /K + -ATPase were declined, suggesting that oxidative damage induced by these intermediates was weakened. According to differential metabolites expression analysis, several key metabolites including aspartate , L-tryptophan, L-asparagine, cytidine, cytosin, stearic acid , behenic acid, were up-regulated, and glutathione , cadaverin, L-2-hydroxyglutaric acid and phytosphingosine were downregulated, clarifying that effective detoxification of atrazine can be performed by UV/bisulfite.