Influence of co-exposure to sulfamethazine on the toxicity and bioaccumulation kinetics of chlorpyrifos in zebrafish (Danio rerio)

Pesticides and antibiotics are frequently present in aquatic environment which may pose potential risks to aquatic organisms. However, the interaction of pesticides and antibiotics in co-exposure model remains unclear. Here, the effects of the co-exposure of sulfamethazine (SMZ) on the toxicity and bioaccumulation of the organophosphorus insecticide chlorpyrifos (CPF) in zebrafish ( Danio rerio ) were explored. The 96-h LC 50 of chlorpyrifos to zebrafish was 1.36 mg/L and sulfamethazine at 1 mg/L slightly increased the acute toxicity with the 96-h LC 50 of 1.20 mg/L which was not significant. The 30-day co-exposure of chlorpyrifos with sulfamethazine at 1 mg/L aggravated the oxidative stress , decreased CarE and AChE activity, and increased CYP450 activity significantly. Furthermore, the co-exposure reduced the accumulation of chlorpyrifos and sulfamethazine while prolonged their depuration duration. The results demonstrated the exposure risk of chlorpyrifos to zebrafish may be enhanced in the presence of sulfamethazine.