Towards the rapid detection of multiple antibiotics in eggs by Surface-enhanced Raman spectroscopy coupled with hollow fiber micro-extraction

Surface-enhanced Raman Spectroscopy (SERS) is an alternative tool in the field of food safety detection. Complicated food sample preparations are the key challenges to use SERS analysis for rapid food test. This study proposed a novel, facile and sensitive hollow fiber liquid-phase micro-extraction coupled with SERS (HF-LPME-SERS) technique for rapid detection of multiple antibiotic residues in eggs samples. The parameters affecting the extraction efficiency were optimized, and the optimized HF-LPME method could extract antibiotics from egg samples directly. That is eased up to sample preparation steps and promotes the SERS to rapid food testing. Moreover, here reported a simple preparation and cost-effective laser induced self-assembly SERS substrate; and applied it to SERS spectra measurement of the extract. The minimum detection concentration was 10 ng/g achieved for multiple antibiotics, which demonstrated the superior SERS performance. The proposed HF-LPME-SERS method provides easy sample operation, low reagent cost and high sensitivity, which would be a green and rapid analytical approach to detect the trace amount of hazardous substances in food.