Super enhancement of methanethiol biodegradation by new isolated Pseudomonas sp. coupling silicone particles

A new strain, Pseudomonas sp. SJ-1, which was able to remove model odorous organics methanethiol (MT) has been isolated from the wastewater treatment plant and identified via 16S rRNA analysis . Initial MT concentration, temperature and pH played an important role in MT removal, and up to 100% of 260 mg L −1 of MT could be removed within 11 h under the optimum conditions (30 °C, pH 7.0) with an average degradation rate of 23.6 mg L −1 h −1 , which was the highest one in literature so far. The silicone particles were added as the non-aqueous phases (NAP) to enhance the performance of MT degradation. The results indicated that the maximum degradation rate and specific cell growth of strain SJ-1 were 2.36 times and 1.31 times by Haldane kinetic model analysis in the NAP added test. The SO 4 2− was identified as the major intermediate and CO 2 as a final product in MT biodegradation. Overall, this is the first report that a newly isolated Pseudomonas sp. could use high concentration MT as sole energy source and carbon source and its activity could be enhanced by adding NAP. The results provide a suggestion for the development of more effective and reliable biological treatment processes.