Fabrication of Ag nanoparticles coupled with ferrous disulfide biocatalyst as a peroxidase mimic for sensitive electrochemical and colorimetric dual-mode biosensing of H2O2

Ag nanoparticles (Ag NPs) with high electrocatalytic activity are of great significance for electrochemical sensors. Nanostructures as scaffold can solve aggregation problem of Ag NPs so that to ensure the stability. In this work, Ag NPs immobilized on FeS 2 nanoprisms (Ag/FeS 2 ) were prepared via an in-situ photocatalytic reduction approach. The enlarged electrode effective surface area, enhanced electron transfer and the synergic impact between Ag NPs and FeS 2 lead to an enhanced catalytic activity. The electrochemical detection method exhibited great performance of low detection limit (0.6 μM), wide linear range and high sensitivity (244.4 μA mM −1 cm −2 ) for H 2 O 2 . Moreover, Ag/FeS 2 as a peroxidase mimic can catalyze oxidation of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to blue product (oxTMB) by H 2 O 2 , which achieved effective detection of H 2 O 2 with a 3.0 μM detection limit. A promising way was successfully eatablished to perform the quantitation of H 2 O 2 in serum and beverage.