Fabrication of anti-oxidant curcumin loaded ceria nanoclusters for the novel delivery system to prevention of selenite-induced cataract therapy in alleviating diabetic cataract

Diabetic cataract (DC) is a major cause of visual impairment for diabetic patients. There are limited range of drug delivery approaches and pharmacologic interventions available to control the diabetic cataract progressions, specifically the eye drops related non-invasive strategies. The reported studies in diabetic cataract designate that non-enzymatic glycosylation and oxidative damages of the crystalline lens played a vital role in the formation cataract pathogenesis. In the present investigation, we developed a novel combination of anti-oxidant curcumin (CUR) and cerium oxide nanoparticles (CeO 2 NPs) entrapped poly lactic-co-glycolic acid (PLGA) copolymeric nanoformulations (CCNPs) to improve auto-regenerative redox and anti-glycation potential to treat diabetic cataract complications. The analytical methods exhibited successful entrapments of curcumin and CeO 2 NPs into the PLGA formulations under X-ray diffraction pattern (XRD), Fourier-transform infrared (FTIR) spectrum and scanning electron microscope (SEM) observations. We investigated and found that prepared CCNPs nanoformulation could have effective anti-oxidant and anti-glycation potential to protect lens epithelial (HLE-B3) cells by prominent prevention of repetitive reactive oxygen species (ROS) generations. In vivo toxicity analysis results indicated that CCNPs have no any inflammation abnormalities and less Ce ions caring on major organs of rat by subcutaneous (SC) injection procedure. We expecting that this developed approach would provide capable therapeutic potential for the treatment of diabetic cataract.