Effect of Melissa officinalis L. Essential Oil Nanoemulsions on Structure and Properties of Carboxymethyl Chitosan/Locust Bean Gum Composite Films

This study aimed to develop active films based on carboxymethyl chitosan (CMCS)/locust bean gum (LBG) films containingMelissa officinalisL. essential oil (MOEO) nanoemulsions. The results showed that the active films incorporated with MOEO nanoemulsion resulted in an increase in the elongation of break, water resistance and improved the film hydrophilicity. Elongation of break increased from 18.49% to 27.97% with the addition of 4% MOEO nanoemulsion. Water resistance was decreased from 56.32% to 25.43%, and water contact angle was increased from 75.13 to 83.86 with the addition of 4% MOEO nanoemulsion. However, the water vapor barrier properties and tensile strength decreased with the addition of MOEO nanoemulsions. The scanning electron microscopic images and Fourier transform infrared spectroscopy results showed that the MOEO was very compatible with the film materials and dispersed evenly in the films. At the same time, the addition of MOEO nanoemulsion significantly enhanced antioxidant and antibacterial activities of C/L-MOEO films. The antioxidant and antimicrobial activities of C/L-MOEO films were increased from 7.16% to 33.81% and 3.52% to 54.50%, respectively. In general, C/L-MOEO film has great application prospects.Keywords:essential oil;active film;nanoemulsions;antibacterial activity