Enhanced denitrification of sewage via bio-microcapsules embedding heterotrophic nitrification-aerobic denitrification bacteria Acinetobacter pittii SY9 and corn cob

In this work, bio-microcapsules were prepared by embedding heterotrophic nitrification and aerobic denitrification (HN-AD) bacteria ( Acinetobacter Pittii SY9) and corn cob. Bio-microcapsules (20 g/L of corn cob and 30% v/v suspension of strain SY9) were porous (pore size 2579.74–3725.44 nm; porosity 53.6%–79.9%). Under the appropriate conditions (C/N > 2, temperature of 20–35 ℃, rotation speed of 100–120 rpm, pH of 7–9), TN removal efficiency of bio-microcapsules reached 94.4%, and 74.0% of nitrogen was converted into N 2 . The results of kinetics fitting indicated that aerobic denitrification was the limiting step during HN-AD process. Bio-microcapsules could slow the carbon release of corn cob for 120 days, which ensuring high HN-AD performance even at low C/N of 2.8. Bio-microcapsule SBR could stably run for 88 days with TN removal efficiency > 90% for synthetic sewage. Bio-microcapsules embedding strain SY9 and corn cob have prospective applications for enhancing denitrification of sewage.