Electrochemical detection of aminoglycoside antibiotics residuals in milk based on magnetic molecularly imprinted particles and metal ions

This work proposed a cost-effective, simple, and highly sensitive magnetic molecularly imprinted particles (MMIPs) electrochemical sensor to indirectly detect kanamycin (KAN), tobramycin (TOB), and gentamicin (GEN). The MMIPs electrochemical sensor was prepared by a complex of metal ions and the MMIPs of rebinding the template, which modified on the magnetic glassy carbon electrode surface. Here, metal ions were used as the signal tracers and amplifiers of the MMIPs electrochemical sensor. Then the response peak currents of metal re-oxidized to metal ion was recorded by differential pulse voltammetry and used to monitor the level of aminoglycoside antibiotics. Under the optimal conditions, the MMIPs electrochemical sensors showed a high sensitivity toward KAN, TOB, and GEN with detection limits of 4.88, 1.28, and 1.07 nmol/L, respectively. Furthermore, the MMIPs electrochemical sensors showed high selectivity for determining KAN, TOB, and GEN, and they were successfully used to detect KAN, TOB, and GEN in milk.