Citric acid cross-linked chitosan for inhibiting oxidative stress after nerve injury

Scaffold design is particularly important and necessary for soft tissue repair such as nerve tissue repair. In this article, we designed and manufactured a macroporous chitosan-based hydrogel with excellent cell compatibility and antioxidant properties. Here, the chitosan (CS) based hydrogel is obtained by repeated freezing and thawing using citric acid (CA) as a cross-linking agent. We have evaluated the effects of citric acid content on the physical and chemical properties of hydrogels through mechanical properties and scanning electron microscopy. CA-CS hydrogel shows a macroporous structure, as the citric acid increases, the mechanical strength increases and the pore size decreases. In vitro cell experiments show that CA-CS hydrogel partakes positive effects on cell survival, adhesion and proliferation, as well as antioxidant properties. All results provide a basis for the construction of porous chitosan-based hydrogels, while demonstrating a promising approach to deal with oxidative stress in nerve injury.