Antibacterial and Fluorescence Staining Properties of an Innovative GTR Membrane Containing 45S5BGs and AIE Molecules In Vitro

This study aimed to add two functional components—antibacterial 45S5BGs particles and AIE nanoparticles (TPE-NIM+) with bioprobe characteristics—to the guided tissue regeneration (GTR) membrane, to optimize the performance. The PLGA/BG/TPE-NIM+membrane was synthesized. The static water contact angle, morphologies, and surface element analysis of the membrane were then characterized. In vitro biocompatibility was tested with MC3T3-E1 cells using CCK-8 assay, and antibacterial property was evaluated withStreptococcus mutansandPorphyromonas gingivalisby the LIVE/DEAD bacterial staining and dilution plating procedure. The fluorescence staining of bacteria was observed by Laser Scanning Confocal Microscope. The results showed that the average water contact angle was 46°. In the cytotoxicity test, except for the positive control group, there was no significant difference among the groups (p> 0.05). The antibacterial effect in the PLGA/BG/TPE-NIM+group was significantly (p< 0.01), while the sterilization rate was 99.99%, better than that in the PLGA/BG group (98.62%) (p< 0.01). Confocal images showed that the membrane efficiently distinguished G+bacteria from G−bacteria. This study demonstrated that the PLGA/BG/TPE-NIM+membrane showed good biocompatibility, efficient sterilization performance, and surface mineralization ability and could be used to detect pathogens in a simple, fast, and wash-free protocol.Keywords:aggregation-induced emission nanoparticles;antibacterial;antibacterial differentiation;bioactive glass;bioprobe;guided tissue regeneration (GTR)