A simple and efficient fluorescent probe for rapidly detecting H2S in GBC - SD cells based on 1,8 - naphthalimide - ebselen

A novel 1,8 - naphthalimide - ebselen fluorescent probe BPHN was synthesized. BPHN has great fluorescence properties: high quantum yield (0.5932), large Stokes shift (125 nm) and remarkable stability at wide pH range (5–11). The results of fluorescence spectroscopy experiments show that BPHN can recognize H 2 S selectively and sensitively. BPHN exhibits low LOD (0.129 μM) and fast response (90 s). The potential of BPHN as a biosensor for H 2 S was demonstrated by imaging of exogenous H 2 S in living cells. BPHN can also detect H 2 S qualitatively and quantitatively in actual water samples.