Selective Removal of Florfenicol from Fetal Bovine Serum by Restricted Access Media–Magnetic Molecularly Imprinted Polymers

Unique restricted access media-magnetic molecularly imprinted polymers (RAM–MMIPs) were prepared as magnetic solid-phase extraction (MSPE) material for florfenicol (FF) and are combined with HPLC–UV to extract FF from fetal bovine serum samples. Under the optimization conditions, the resulting materials had a uniform imprinted layer, acceptable adsorption amounts (22.92 mg/g), rapid binding kinetics (80 min) and satisfactory selectivity. In addition, RAM–MMIPs presented satisfactory recoveries (95.6–102.1%), relative standard deviation (RSD) (2.7–3.9%) and lower limits of detection (LOD) (10.5 μg/L). The method was applied to the real samples. Compared with other reported methods, this method exhibits the advantages of simple, fast and, efficient operation, using a relatively small amount of the RAM–MMIPs adsorbent. The developed method can enrich FF from fetal bovine serum samples. Graphic abstract