Production and characterization of antimicrobial bacterial cellulose membranes with non-leaching activity

Kanamycin covalently grafted onto regenerated bacterial cellulose (RBC) membranes were constructed to prepare novel antimicrobial BC membranes. The prepared kanamycin grafted RBC membrane exhibits nanoporous three-dimensional network structure with a large surface area and high porosity. The antibacterial activity was explored and the result shows that the kanamycin grafted RBC membrane displays excellent antibacterial activity towards E. coli , C. albicans and S. aureus without any kanamycin leaching. Furthermore, MTT assay was carried out and the result demonstrates that the kanamycin grafted RBC membrane displays good biocompatibility.