Fabrication of large size individual octahedral tungsten oxide hydrate and Au NPs as SERS platforms for sensitive detection of cytochrome C

Surface-enhanced Raman scattering (SERS) has attracted much attention with its powerful trace detection and analysis capabilities, especially biological and environmental molecules. However, building a protein SERS detection platform based on semiconductor devices is a huge challenge. Herein, through the synergy of NH 3 and nickel foam, a large-sized semiconductor tungsten oxide hydrate platform (WOHP) was synthesized. The crystal plane of a single WOHP particle is larger than the excitation spot. As a SERS substrate, WOHP can make full use of the excitation light without destroying the structure during the protein molecules detection process. Through the synergy of WOHP and Au NPs, the enhancement factor is 1.5 × 10 4 . Raman peaks of WOHP can be used as references for the detection of typical protein cytochrome C (Cyt C). As the Cyt C concentration decreases, the I Cyt C /I WOHP ratio decreases, and the signal can still be obtained when the concentration is as low as 5 × 10 −9  mol L −1 . More importantly, the method does not affect the catalytic activity of Cyt C and can be applied to the detection of Cyt C concentration in serum.