Metabolite pattern in root nodules of the actinorhizal plant Casuarina equisetifolia

Casuarina equisetifolia L. (Casuarinaceae), an actinorhizal plant , exhibits mutualistic symbiosis with Frankia and promotes nitrogen fixation in root nodules . While the exchange of metabolites between host plant and microsymbiont is well understood in legume symbioses, the situation in the symbiosis between nitrogen-fixing Frankia and actinorhizal plants is less clear. In this study, a metabolomic approach was applied to root nodules of mature C. equisetifolia trees, leading to the identification of an undescribed taraxerane-type triterpenoid ester, 3- O -dihydrocoumaroyl β -taraxerol, along with twelve known compounds. An abundant component was tyramine with a content of 2.76 ± 0.315 mg/g FW in mature nodules. Tyramine specifically and abundantly accumulated in mature nitrogen-fixing nodules compared to senescent nodules, stems, leaves, and seeds. In addition, the potential function of tyramine was preliminarily examined and discussed.