Enzyme-induced multicolor colorimetric and electrochemiluminescence sensor with a smartphone for visual and selective detection of Hg2+

In this study, we developed a novel dual-analytical platform for the visual, sensitive, and reliable analysis of mercury ions (Hg 2+ ) in environmental water samples. Importantly, thymine (T)-rich DNA probes were utilized to form T-Hg 2+ -T base pairs in the presence of Hg 2+ to ensure the specificity of the method. We synthesized new luminescent tris(4,4′-dicarboxylicacid-2,2′-bipyridyl) ruthenium (II) dichloride (Ru(dcbpy) 3 2+ )-modified metal-polydopamine frameworks ( [email protected] ), which were then applied to construct an electrochemiluminescence (ECL) system for the first time, and it achieved accurate and sensitive quantitative detection of Hg 2+ . To achieve rapid on-site determination, a multicolorimetric system based on a smartphone was established by inducing deposition of silver shells on gold nanorods (Au NRs). Under optimized conditions, the dual-modal assay showed an excellent response for Hg 2+ in the linear range of 2 pmol L −1 to 500 nmol L −1 , with a low detection limit of 0.32 pmol L −1 . Moreover, the proposed method demonstrated satisfactory selectivity , stability, and acceptable reproducibility for the detection of Hg 2+ . The recovery of lake water samples ranged from 98.53% to 111.97% for the ECL method and from 95.04% to 106.11% for the colorimetric method, indicating the potential applicability of the proposed method for monitoring environmental water samples.