Multi-Enzyme-Synergetic ultrathin protein nanosheets display high efficient and switch on/off antibacterial activities

Natural enzymes are expected to be used in biomedical fields due to their extensive antibacterial activity, good biocompatibility and resistance to bacteria. Herein, we constructed a multi-enzyme protein assembly with ultrathin single protein sheet structure by the strategy of interface assembly and developed an efficient and intelligent antibacterial system based on the combination of multi-enzymes. The multi-enzyme protein assembly not only displayed extremely high antibacterial activity, but can also be turned on and off through the denaturant induced folding and unfolding of natural enzymes, and this process can be realized for several reversible cycles. Such an intelligent antibacterial material provides the possibility for its application in biomedical and other fields.