Schiff base network-1 incorporated monolithic column for in-tube solid phase microextraction of antiepileptic drugs in human plasma

A novel monolithic column incorporated with Schiff base network-1 (SNW-1) was prepared for in-tube solid phase microextraction (SPME) of antiepileptic drugs . The SNW-1 nanoparticles was synthesized by a solvent thermal method and embedded in the poly(glycidyl methacrylate-ethylene glycol dimethacrylate) (GMA-EGDMA) monolithic column by in situ polymerization. The amount of SNW-1 in polymerization solution for preparation of monolithic column was optimized. Fourier transform infrared spectrometry and scanning electron microscope were used to characterize the obtained monolithic column. The SNW-1-monolithic column was connected to the high-performance liquid chromatography (HPLC) system and applied for online extraction and analysis of antiepileptic drugs. The extraction conditions including sample solution flow rate , desorption time, acetonitrile content, pH of sample solution and sample volume were investigated. Under optimal conditions, the proposed SPME-HPLC method showed high extraction efficiency, good linearity, low limits of detection (0.2 ng/mL) and good repeatability (relative standard deviations < 2.41%). This method was also applied to the analysis of antiepileptic drugs in human plasma samples and the recoveries were in the range of 88.6–106.1% with RSDs less than 3.51%.