Eco-friendly ionic liquid imprinted polymer based on a green synthesis strategy for highly selective adsorption tylosin in animal muscle samples

A novel eco-friendly molecularly imprinted polymer (MIP) was proposed as solid-phase extraction (SPE) adsorbent to selective adsorption tylosin (TYL) in animal muscle samples. The MIP was synthesized in aqueous by using 1,4-butanediyl-3,3-bis-1-vinyl imidazolium chloride and 2-acrylamide-2-methylpropanesulfonic acid as bifunctional monomer. The obtained MIP had excellent selectivity towards TYL in water, and the maximum binding capacity can reach 123.45 mg g −1 . Combined with high-performance liquid chromatography, the presented MIP can be used as SPE sorbent to recognize and detect TYL in the range of 0.008 to 0.6 mg L −1 ( R 2  = 0.9995). The limit of detection and limit of quantification were 0.003 mg L −1 and 0.008 mg L −1 , and the intraday and interday precision were 1.05% and 3.36%, respectively. Under the optimal condition, the established MIP-SPE-HPLC method was successfully applied to separate and determine trace TYL in chicken, pork, and beef samples with satisfactory recoveries ranged from 94.0 to 106.3%, and the MIP-SPE cartridge can be cycled at least 20 times. This study implies a promising green MIP-SPE-HPLC method for highly selective adsorption and analysis trace TYL in complex matrices.