Sandwich-type electrochemical immunosensor for CEA detection using magnetic hollow Ni/C@SiO2 nanomatrix and boronic acid functionalized CPS@PANI@Au probe

A sandwich-type electrochemical immunosensor was developed for the detection of CEA where hollow magnetic silica coated nickel/carbon (Ni/ [email protected] 2 ) nanocomposites was used as an immobilized carrier and gold nanoparticle-coated PANI microsphere ( [email protected] @Au) as electrochemical transducer. Magnetic assembly of Ni/ [email protected] 2 nanocomposites allow easy separation and assembly which eliminates the further modification process. In addition, the prepared [email protected] @Au possess good biocompatibility, and electrical conductivity. The fabricated immunosensor show excellent sensing performance including a wide dynamic range from 0.006 to 12.00 ng mL −1 and low detection limit at 1.56 pg mL −1 . Moreover, this sensor shows superior selectivity with excellent reliability and reproducibility which might lead into easier implementation in clinical setting.