DNA–Gold Nanozyme-Modified Paper Device for Enhanced Colorimetric Detection of Mercury Ions

In this work, a paper device consisted of a patterned paper chip, wicking pads, and a base was fabricated. On the paper chip, DNA–gold nanoparticles (DNA–AuNPs) were deposited and Hg2+ions could be adsorbed by the DNA–AuNPs. The formed DNA–AuNP/Hg2+nanozyme could catalyze the tetramethylbenzidine (TMB)–H2O2chromogenic reaction. Due to the wicking pads, a larger volume of Hg2+sample could be applied to the paper device for Hg2+detection and therefore the color response could be enhanced. The paper device achieved a cut-off value of 50 nM by the naked eye for Hg2+under optimized conditions. Moreover, quantitative measurements could be implemented by using a desktop scanner and extracting grayscale values. A linear range of 50–2000 nM Hg2+was obtained with a detection limit of 10 nM. In addition, the paper device could be applied in the detection of environmental water samples with high recoveries ranging from 85.7% to 105.6%. The paper-device-based colorimetric detection was low-cost, simple, and demonstrated high potential in real-sample applications.Keywords:paper device;signal enhancement;mercury ion;colorimetric detection