Effects of interaction between enrofloxacin and copper on soil enzyme activity and evaluation of comprehensive toxicity

Antibiotics are detected in association with heavy metals in the soil. However, interactions between antibiotics and heavy metals on soil enzyme activity have yet to been studied thoroughly. In this study, soil enzyme activity (urease, sucrase, phosphatase , and Rubisco) were measured after exposure to soils dosed with copper (Cu) and/or enrofloxacin (ENR) over 28 days. Enzyme responses to ENR only treatments varied, but Cu exhibited a strong negative response from all soil enzymes except Rubisco. An interaction between the effects of the two pollutants on soil enzymes was observed in the combined contamination treatments. Greater comprehensive toxicity to soil enzyme activity was observed in combined treatment groups compared to other groups. We anticipate our studies can provide a scientific theoretical basis for the combined pollution of antibiotics and heavy metals in soil.