Luteimonas wenzhouensis Sp. Nov., A Chitinolytic Bacterium Isolated from a Landfill Soil

A Gram-staining-negative, aerobic, non-motile, rod-shaped bacterium with degradation ability of chitin, designated strain YD-1  T , was isolated from landfill soil sample collected in Wenzhou, Zhejiang province, China. The growth of strain YD-1  T occurred optimally in the tryptone soy broth (TSB) with 1.0% NaCl at pH 7.0–8.0, 30 °C. Ubiquinone-8 (Q-8) was the predominant quinone. The polar lipids of strain YD-1  T consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, five glycolipids and four lipids. The major fatty acids were iso-C 15:0 (30.7%), iso-C 17:1 ω9c (23.2%), iso-C 11:0 (18.9%), iso-C 11:0 3-OH (6.8%) and iso-C 17:0 (5.9%). Phylogenetic analysis based on the 16S rRNA gene sequences indicated that strain YD-1  T was affiliated to the genus Luteimonas with the highest similarity to Luteimonas marina KCTC 12327  T (97.3%), followed by Luteimonas aquatica DSM 22088  T (96.5%) and Luteimonas composti CCUG 53595  T (96.4%). The genomic DNA G + C content of strain YD-1  T was 71.8 mol%. Average nucleotide identity (ANI) and the digital DNA–DNA hybridizations (dDDH) for draft genomes between strain YD-1  T and Luteimonas marina KCTC 12327  T were 82.7% and 26.1%, respectively. On the basis of genotypic, phenotypic and chemotaxonomic data, strain YD-1  T is considered to represent a novel species to degrade chitin in the genus Luteimonas , for which the name Luteimonas wenzhouensis sp. nov. is proposed, with YD-1  T (= KCTC 72425  T  = CCTCC AB 2019153  T ) as the type strain.