Sodium 4-styrenesulfonate functionalized nanofibers mat as 96-well plate solid–phase extraction adsorbent for quantitative determination of multiple β-agonists residues in pork samples

In this work, sodium 4-styrenesulfonate functionalized polyacrylonitrile nanofibers mat (SS/PAN NFM) was firstly prepared and applied as 96-well plate solid–phase extraction adsorbent for quantitative determination of seven β-agonists residues in pork samples. The functional modification endowed the SS/PAN NFM with superior adsorption performance for target β-agonists. The adsorption process is spontaneous (Δ G  < 0), the initial adsorption rate can reach 6.03–9.09 mg/g/min and the maximum adsorption capacity is calculated to be 48.3 mg/g at 298 K. Moreover, SS/PAN NFM can be reused for 12 times without degradation in adsorption capability. Combined with UPLC–MS/MS, the limits of detection can reach 0.006–0.24 μg/kg, the recoveries ranged from 87.2% to 111% and the relative standard deviations of intra-day and inter-day precisions were in the scope of 1.75%–11.6% and 5.08%–13.5%, respectively. The obtained results fully demonstrated the practicability of this method in preventing the hazard of β-agonists residues.