High biocompatible AuNCs-silk fibroin hydrogel system for visual detection of H2O2

Hydrogen peroxide (H 2 O 2 ) quantification in biomedicine is an important indicator for different biological tissues. In this study, we developed a visual detection for H 2 O 2 sensing based on gold nanozyme-silk fibroin (AuNCs-SF) hydrogel hybrid system with good compatibility between silk fibroin hydrogel and BSA-AuNCs. The AuNCs-SF hydrogel exhibited a fast response to H 2 O 2 directly by fluorescence change and showed high sensitivity (limit of detection of 0.072 mM) and well stability. Moreover, the hydrogel possessed high selectivity for H 2 O 2 in FBS solution and excellent biocompatibility to L929 cells (mouse fibroblasts). Therefore, the AuNCs-SF hydrogel hybrid system has a great potential in the fields of clinical diagnosis for visual detection of H 2 O 2 .