A photostable fluorescent probe based on PET off for the detection of hydrogen sulfide and its application in bioimaging

As an important small gas signal molecule, H2S has become an important research hotspot for both chemists and biologists. Therefore, we designed and synthesized a benzene-conjugated coumarin connected to 2,4-dinitrobenzene via an oxygen atom (P1) to detect H2S and explored its applications in biological imaging. After 40 minutes of long-time irradiation and different pH tests, we found that P1 has good photostability and chemical stability, indicating that P1 had the ability to detect H2S accurately. In addition, P1 has a fast response speed, low detection limit and low toxicity. Based on these characteristics, P1 was used to detect H2S in live cell imaging.