Physiochemical property and antibacterial activity of formulation containing polyprenol extracted from Ginkgo biloba leaves

Polyprenol is an important active ingredient in Ginkgo biloba leaves (GBL) with great industrial application potential. In this paper, a novel high energy ball milling extraction technology coupled with silver-thiolate material purification method was developed, with which a high purity of GBL polyprenol (GBP) was obtained. Results showed that this method had a high extraction efficiency, and GBP of a purity up to 99.8 % was produced. GBP liposomal gel (GLG) was prepared, and its stability, rheological property, release behavior and antibacterial activity were evaluated. According to the results, GLG had a small particle size and high homogeneity which was desirable for topical application, and it also manifested good physiochemical stability and suitable plastic flow property. In addition, GLG illustrated excellent inhibitory effect against Staphylococcus aureus , Escherichia coli , Candida albicans and Streptococcus pneumoniae , and the minimal inhibitory concentration was 25∼200 μg/mL. The systematic research provided a useful method for the biorefining of GBL.