Rapid determination of marbofloxacin by surface-enhanced Raman spectroscopy of silver nanoparticles modified by β-cyclodextrin

A novel surface enhanced-Raman spectroscopy (SERS) assay for marbofloxacin was developed based on β -cyclodextrin-modified silver nanoparticles ( β -CD-AgNPs). The marbofloxacin could interact with β -CD-AgNPs and a new assembly was formed by Ag N covalent bond . This assembly was characterized by the spectra of FT-IR and UV–vis. The optimal measurement conditions were studied in detail. In 0.033 mol L −1 HCl solution, marbofloxacin had a sensitive SERS signal at 806 cm −1 . The enhancement factor (EF) was 2.11 × 10 7 . There was a good linear correlation between the concentration of marbofloxacin and SERS intensity: the linear range was 0.003–0.03 μmol L −1 ( r 2  = 0.996). The limit of detection (LOD) (S/N = 3) was 1.7 nmol L −1 (S/N = 3). Moreover, the influence of some interferences including Cu 2+ , K + , Zn 2+ , Ca 2+ , Na + , Mg 2+ , glucose and tiamulin on the determination were studied. The developed SERS method was used to detect the content of marbofloxacin in chicken and duck, the recovery was 101.3%–103.1% with RSD 4.07%–6.83%.